angioquant version 1.33  (MathWorks Inc)

Journal: bioRxiv

Article Title: Impact of hypoxia induced VEGF and its signaling during caudal fin regeneration in Zebrafish

doi: 10.1101/105767

Figure Lengend Snippet: Statistical analysis of fold change in blood vessel documented using angioquant software:Increase in (A 1, 2, 3) length, (B 1,2,3) size and (C 1,2,3) Junction of blood vessel of the dorsal, cleft and ventral region in regenerated tail fin under hypoxia condition observed at 0dpa, 3dpa, 5dpa and 10dpa. At 3 dpa size of blood vessel increased by 1.4 fold in at dorsal, 1.3 fold in cleft and 1.5 fold in ventral regions. At 5dpa Size of the blood vessel showed fold increase of 1.8 times in dorsal region, 1.6 times in cleft and 1.7 in dorsal region of the caudal fin. Blood vessel length were found a fold increase of 2.3 in dorsal region 2.2 in cleft and 1.9 in ventral region, compared to normoxia, lacking signs of blood vessel development in regenerated tissue. at 10 dpa is significantly superior to control demonstrating a fold increase of 3.6 in length, 2.8 in junction and 2.6 in size (Fig.2) of the dorsal region compared to cleft region with fold increase at 3.5in length, 2.7in junction, 2.3 in size and ventral region with 2.8 in length, 2.7 in size and 2.1 in size of the blood vessel. The fold change is expressed as mean ± SEM. Statistical analyses performed using one-way ANOVA followed by Turkey’s multiple comparison tests for comparison between treatment values and control. P values < 0.05 considered to be statistically significant. ** P value <0.005, *** P value < 0.001, **** P value < 0.0001.

Article Snippet: Blood vessels were quantified in terms of length, junction and sprouting using angioquant software (Version 1.33, MathWorks).

Techniques: Software, Control, Comparison